the two-way ANOVA that incorporated the averaged data from post-vapor sessions 1-3, a two-way ANOVA was conducted that only used the final session prior to escalation instead of the averaged data. When the two-way mixed model ANOVA was used to v analyze the post-vapor self-administration data, that included the averaged data, from animals challenged with acute FN-439 or aCSF infusions prior to and after self-administration sessions during acute withdrawal (see Figure 4), a significant main effect of Drug Treatment and a significant Drug Treatment × Session interaction were observed (F (1, 9) = 6.520, p≤0.05 and F (1, 9) = 6.980, p≤0.05, respectively). The two-way ANOVA that utilized the single post-vapor session data prior to escalation from animals challenged with acute FN-439 or aCSF infusions identified that the Drug Treatment × Session interaction (F (1, 9) = 12.347, p≤0.01) was maintained. Post-hoc independent samples t-tests comparing the performance of animals during the escalation session following the acute FN-439 vs. aCSF-treatments were significant (t (9) = 2.861, p < 0.05). These results showed that FN-439 and aCSF differentially affected the dependent animals and that the aCSF-treated group showed escalated self-administration when compared to the FN-439 treatment group.
