Chunk #9 — Methods — RNA isolation

Source

Transcriptome analysis of alcohol-treated microglia reveals downregulation of beta amyloid phagocytosis.

Embedded

yes

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RNA was isolated using Direct-zol RNA MicroPrep (Zymo Research, Irvine, CA, USA) according to instructions. The resulting RNA quality was determined using the 4200 TapeStation Instrument (Agilent, Santa Clara, CA), and all samples had RNA integrity numbers (RIN) above 8.