To adapt iPSCs to a defined medium culture, early MR31 and MMW2 lines (at passages 10–15) were continuously cultured in StemPro medium (a chemically defined medium) on a defined substrate (CellStart) for over 10 passages. As seen in Figure 1A–1D, like hESCs, both iPSC lines grown in defined medium expressed pluripotentcy markers such as Oct4, TRA-1-60 and SSEA4, and maintained a normal karyotype in prolonged culture. No differences were observed in the course of adaption regarding morphological characteristics between hESCs and iPSCs. As the results are similar for both iPSC lines, staining images of only one of the lines (MR31) are shown here.
