Chunk #55 — Online Methods — Single-nuclei RNA-seq Assay — Cell ranger Count Analysis

Source

Integrated Single-Cell Multiomic Profiling of Caudate Nucleus Suggests Key Mechanisms in Alcohol Use Disorder.

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Cell Ranger Count (cellranger-count-7.0.1, http://support.10xgenomics.com/) was utilized to process the raw sequence data derived from the single-cell multiome libraries. The gene expression FASTQ files were processed with the cellranger count algorithm. The reference refdata-cellranger-GRCh38-2020-A (10x Genomics) was used. The filtered gene-cell barcode matrices and fragment files were used for further analysis.