Chunk #77 — PROCEDURE — Functional testing: detection of genomic microdeletions by PCR ● TIMING 3–4 h hands-on; 2–3 weeks expansion

Source

Genome engineering using the CRISPR-Cas9 system.

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yes

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(A) Deletion or microdeletion analysis For the analysis of microdeletions, use the Out-Fwd and Out-Rev primers, both of which are designed to anneal outside of the deleted region, to verify the successful deletion by product size analysis. If the deletion size is more than 1 kb, set up a parallel set of PCRs with In-Fwd and In-Rev primers to screen for the presence of the WT allele (Fig. 5c).