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Chunk #5 — RESEARCH DESIGN AND METHODS — Temperature measurements.

Source
Nicotine induces negative energy balance through hypothalamic AMP-activated protein kinase.
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yes

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Body temperature was recorded with a rectal probe connected to digital thermometer (BAT-12 Microprobe-Thermometer; Physitemp, Clifton, NJ). Skin temperature surrounding BAT was recorded with an infrared camera (E60bx: Compact-Infrared-Thermal-Imaging-Camera; FLIR, West Malling, Kent, U.K.) and analyzed with a specific software package (FLIR-Tools-Software; FLIR). We used eight animals per group, and for each animal, three or four pictures were analyzed. The skin temperature surrounding BAT for one particular animal was calculated as the average temperature recorded by analyzing those pictures.