To intersect the genes found to be differentially expressed in OUD within females and males, we also compared to differentially expressed genes identified by microarray studies of striatum of male mice exposed to drugs104. Piechota et al. identified from hierarchical clustering 2 main gene signatures that became pronounced time-lapsed since drug exposure, A and B, with subsets of the second B1, B2, and B3. We identified the human orthologs of these gene sets and performed GSEA as above on the differentially expressed genes identified across the full cohort, on the subset of female and male individuals, or the caudate and putamen samples. We report the main findings in the text and the full set of enrichments in Supplementary Data I–S16.
