Chunk #26 — Methods — Neuronal culture screen

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Ultrasensitive fluorescent proteins for imaging neuronal activity.

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Neurons were stimulated in imaging buffer containing a drug cocktail to inhibit synaptic receptors (10 μM CNQX, 10 μM (R)-CPP, 10 μM gabazine, 1 mM (S)-MCPG, Tocris). Under these conditions, intracellular calcium increases are presumably caused by the opening of voltage sensitive calcium channels.