A set of genes with promoter DNA methylation changes during differentiation as well as by alcohol, i.e. genes altered during differentiation and affected by alcohol (changed both in control differentiated vs. control undifferentiated, and in control differentiated vs. alcohol differentiated) and related to the nervous system, were validated using Sequenom MASSarray technology. Overall, based on the two criteria in the Methods, we validated 12 genes out of 26 tested from the MeDIP-microarray results. Their methylation alteration site is shown in Table 1. Examples of comparison of MeDIP-ChIP and Sequenom is shown in Figure 5.
