To facilitate construction of a fragment file outside of running the 10x Genomics Cellranger software, we developed a Python package (Sinto) capable of generating the fragment file from a BAM file. This software is available on the Python Package Index (https://pypi.org/project/sinto/), Bioconda (https://anaconda.org/bioconda/sinto), and GitHub (https://github.com/timoast/sinto).
