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Chunk #13 — Common Techniques of Gene Expression Profiling — High-throughput sequencing or RNA-Seq

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Gene expression in the human brain: the current state of the study of specificity and spatiotemporal dynamics.
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At present, RNA-Seq, dubbed “a revolutionary tool for transcriptomics” (Z. Wang, Gerstein, & Snyder, 2009), is the most promising and increasingly used technique in global gene expression studies. Although still few, transcriptome studies of the human brain have been featured in a recent review that discussed how high resolution high-throughput sequencing techniques might increase our knowledge of gene expression in such complex organs as the brain (van der Brug, Nalls, & Cookson, 2010).