Astrogenesis is another essential facet of corticogenesis. We examined the presence of astrocytes in the CP-like area of hCOs after longer culture (day 105) as reported before (Pasca et al., 2015). Importantly, we found that NeuN+ cells in the CP-like area were extensively intermingled with GFAP+ processes from astrocytes (Figure 3M). Similarly, neuronal processes stained by MAP2 were commingled with GFAP+ glia processes in the CP-like area (Figure 3N). These data indicate astrocytes were generated and intertwined with differentiated neurons as hCOs developed.
