release of GENCODE). Considering the depth of reads of the HBM data (averaging over a billion read depth) from the 16 different tissues, we have not identified many missing coding genes based on PhyloCSF. Indeed, since 3127 HBM Ensembl genes consist of only two exons, it is highly likely these constitute new lncRNAs we have not yet annotated and will be merged into a later release of GENCODE.
