We next examined whether the dendrites present within the microgrooves possess spines, the sites of excitatory synaptic connections. When neurons were infected with an RFP-Sindbis virus, numerous protrusions with spine-like morphologies were evident extending from dendrites within the microgrooves (Figure 1D). Together, these data shows that dendrites grow and extend considerable distance in the microgrooves; many of these dendrites possess spines.
