at a 70% saturation rate, detecting an average of 3513 ± 77 genes (mean ± standard error) and 13,635 ± 560 unique transcripts per nucleus per individual (Fig. 1c). The average number of genes detected and the number of unique mapped identifiers for transcripts were consistent between unaffected individuals and individuals with OUD (p = 0.55, linear regression, Supplementary Data 1-S1).
