Cells were differentiated as described above. Standard patch clamp recordings were carried out using an extracellular solution (in mM) consisting of 115 NaCl, 2 KCl, 3 CaCl2, 10 glucose, 1.5 MgCl2 and an intracellular (in mM) consisting of 140 potassium gluconate (C6H11KO7), 5 KCl, 2 MgCl2, 10 HEPES, 0.2 EGTA, 2.5 Na-ATP, 0.5 Na-GTP, 10 Na2phosphocreatine. Voltage activated currents were recorded in the voltage clamp configuration using an Axopatch 200B patch clamp amplifier and head stage. Series resistance was compensated up to 80%. Action potentials were recorded in the current clamp configuration on the Axopatch 200B. Data was acquired using pClamp10 at a rate of 10 kHz filtering at 5 Hz. Data was analyzed using pClamp software and custom scripts written in Igor (Wavemetrics).
