Chunk #47 — METHODS — Gene expression analysis

Source

Modelling schizophrenia using human induced pluripotent stem cells.

Embedded

yes

Text

For qPCR, cDNA was synthesized using Superscript III at 50°C for one to two hours, inactivated for 15 minutes at 70°C and then treated with RNAaseH for 15 minutes at 37°C, inactivated with EDTA and heated to 70°C for 15 minutes. qPCR was performed using SybrGreen. Primers used are listed in SI Table 8.