Chunk #32 — Materials and Methods — SNP selection for genotyping

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A systematic mapping approach of 16q12.2/FTO and BMI in more than 20,000 African Americans narrows in on the underlying functional variation: results from the Population Architecture using Genomics and Epidemiology (PAGE) study.

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three HapMap samples (CEU, YRI and HBC/JPT). SNPs were excluded if (a) the Illumina design score was <0.5 or (b) there were SNPs within 15 bp in both directions of the SNP of interest with allele frequency of >0.02 among Europeans (CEU). SNPs annotated as nonsynonymous, essential splice site, or stop codon were included regardless of allele frequency, design score, or nearby SNPs in the primer.