Using this FHS sample, we performed in silico replication of the nine interesting SNPs identified in our GWAS cohort (see details for these nine SNPs in the Results section). Genotyping of these nine SNPs in the FHS sample was performed with Affymetrix 500K mapping array plus Affymetrix 50K supplementary array. For details of the genotyping method, please refer to Framingham SHARe at NCBI dbGaP website (http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000007.v3.p2). Specifically, for the nine SNPs of interest, the call rates are as follows: 98.98% for rs12505771, 99.10% for rs6838494, 98.58% for rs17354547, 99.14% for rs17354568, 98.84% for rs17007301, 98.85% for rs1402812, 98.41% for rs4956396, 98.60% for rs13133830, and 98.33% for rs4956302. The p values for HWE test at these nine SNPs range from 0.41 to 0.88, suggesting HWE and good genotyping quality at these SNPs.
