of Medicine policies for non-human primate environmental enrichment. Animal care procedures strictly followed the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committees of Emory University and Wake Forest School of Medicine. Monkeys were sacrificed and necropsied on average at age 6.2 years (range between 3.6 and 8.2 years old) after the six-month treatment protocol by an overdose of barbiturate and transcardially perfused with ice cold saline. The brains were removed and cut into 4 mm slabs in the coronal plane using a brain matrix (EMS, Fort Washington, PA) and immediately frozen and stored at −80°C. Tissue was dissected from slabs of the right hemisphere that included the basal ganglia from the rostral pole to the beginning of the anterior commissure. The DLPFC was dissected from the dorsal and ventral banks of the principal sulcus (Area 46) and pulverized. The identical RNA-seq protocol (using the RiboZero Gold kit [Illumina]) was followed as for the primary human CMC cohort. Sequencing data were processed similarly as for the human CMC cohort, with reads aligned to the macaque reference genome and transcriptome (mmul1), but with two minor changes:
