5 %; P = 1.6 × 10−7). The region harboring this SNP is displayed in Supplemental Figure S3 (Pruim et al. 2010). Supplemental Table S2 contains the top SNPs associated with initiation at P < 1 × 10−5. Table 1 contains the five genes showing the strongest association signal with initiation along with their functions (according to gene ontology (GO) annotations Ashburner et al. 2000).Table 1Top five genes showing the strongest association with initiation of cannabis useGene name (Gene ID)ChrStart positionNumber of SNPs assigned to geneKey SNPs position (rs number)Gene featureKey SNPs P-valueGene P-valueMolecular function according to gene ontology annotationZinc finger protein 181 (ZNF181)1935225479235221228 (rs35487050)Upstream1.6 × 10−7 3.7 × 10−6 Nucleic acid binding; metal ion bindingmicroRNA 643 (MIR643)19527850491052787471 (rs2434422)Intronic3.7 × 10−6 3 × 10−5 –52788044 (rs321908)Intronic8.5 × 10−6 –Zinc finger protein 766 (ZNF766)19527728234152787471 (rs2434422)Intronic3.7 × 10−6 1.1 × 10−4 Nucleic acid binding; metal ion binding52788044 (rs321908)Intronic8.5 × 10−6 –52770905 (rs57523152)Upstream3.3 × 10−5 –52790542 (rs139570481)Intronic2.3 × 10−4 –52792311 (rs147711278)Intronic3.4 × 10−4 –52775301 (rs2089275)Intronic1 × 10−2 –Phosphatidylinositol-specific phospholipase C, X domain containing 2 (PLCXD2)311139352260111416310 (rs1355767)Intronic1.1 × 10−6 1.1 × 10−4 Phosphoric diester hydrolase activity111399209 (rs7651713)Intronic1.2 × 10−6 –111460129 (rs57628489)Intronic1.3 × 10−2 –111430969 (rs16858448)Intronic1.5 × 10−2 –111438443 (rs12637233)Intronic1.5 × 10−2 –111479048 (rs7643067)Intronic1.6 ×
