DRD1, RXFP1 and CPNE4 on an adjacent section because of overlapping channels in the OPRM1 and RXFP1 probes. The locations of RXFP1 and CPNE4 islands in the OPRM1 and DRD1 double stained sections were determined by adjacent section labeled with RXFP1 and CPNE4. The quantification of OPRM1 grains numbers was similar to ARHGAP6 or GREB1L quantification except that we used overexposed DRD1 to draw ROIs for each cell.
