suggesting that an active RNA trafficking mechanism exists for both isoforms (Fig. 2A). Higher levels of Girk2c mRNA relative to Girk2a mRNA were detected, with the difference reaching statistical significance in neuropil (P = 0.04) but not cell bodies (P = 0.07). Interestingly, we detected considerable read coverage past the annotated terminal exon for both transcripts, suggesting transcription occurs past the annotated 3′ ends in hippocampal pyramidal neurons.
