due to evaporation in unsealed culture dishes mimics a pattern of ethanol exposure more similar to human drinkers (Lieberman, Levine, Kranzler, Abreu, & Covault, 2012). Evaporation of ethanol was examined in 6-well culture dishes with culture media by measurement of ethanol concentrations (0, 40, and 75 mM) at 0, 12, and 24 h using an AM1 Alcohol Analyzer (Analox Instruments, Ltd.). Five independent 6-well culture dishes were used for analysis. The calculation of half-life was performed as previously described (Chen, Ezzeddine, & Shyu, 2008). Briefly, results were plotted as the log of the fraction remaining, normalizing to the initial (T = 0) concentration. The linear regression fit is plotted as a line, and the half-life was calculated from the slope of the fit, as −log(2)/slope.
