Insertions/deletions within the genomic length of Hs04090007_cn (Chr.22:19,318,224-19,419,219) were analyzed by real-time genomic quantitative PCR using the TaqMan Copy Number Assay (Applied Biosystems). For the analysis of copy number variant in the 22q11.2 region, the RNaseP gene was used as a normal copy number control gene. No copy number polymorphisms have been documented in RNaseP genes. For genomic quantitative PCR, DNA solutions were first measured using an ultraviolet spectrophotometer and further quantified using a TaqMan RNase P Detection Reagent kit (Applied Biosystems).
