Flow cytometry analysis was performed at Day 35 of GABAergic interneuron differentiation in order to assess the purity of the cell population. Cells were dissociated and fixed with 4% paraformaldehyde and stained with the following antibodies: NKX2.1 (sc-13040, Santa Cruz Biotechnology), and GABA (A2052, Sigma). The secondary antibody was IgG-conjugated Alexa Fluor 488 (Life Technologies). Live and dead cells were identified using the Zombie UV Fixable Viability Kit (Biolegend). Unstained as well as IgG-conjugated Alexa Fluor 488 (Life Technologies) secondary antibody alone was used as a control and to gate positive populations. Cells were analyzed on a BD LSR II Flow Cytometer (BD Biosciences), and data were interpreted with FlowJo Version 10.
