Subsequently, DEGs between NP and E neurons, as well as NP and L neurons, were identified and processed for hierarchical clustering of gene expression, which illustrated close assembly of E and L neuron expression profiles separating from those of iPSCs and NPs (Fig 4B). BPD_E_3 was an apparent outlier. Without technical replicates it is not possible to determine if this variation comes from culture effects, clonal variation or inherent differences between patients. There was no clear segregation of BPD and control cells. This was consistent with the lack of morphological differences between BPD and control cells.
