Three to 4 animals per group were used in all microarray and quantitative immunoprecipitation experiments. An additional cohort of 8 animals per group was used for gene expression analysis. A maximum of 2 animals from any one litter were used, to control for possible effects attributable to variation between litters rather than variation as a function of High and Low LG. The animals were Long-Evans hooded rats born in our colony originally obtained from Charles River Canada (St. Constant, Québec). Maternal behavior was scored by using a version of the procedure described elsewhere[16]. Hippocampal tissue was dissected from 90-day-old (adult) male High and Low LG offspring and stored at −80°C. Genomic DNA extraction (DNeasy, Qiagen) and quantification (Nanodrop ND-1000 spectrophotometer, Thermo Scientific) as well as RNA extraction (RNeasy plus, Qiagen) and quality assessment (Bioanalyzer 2100, Agilent) were performed according to the manufacturer's protocol (see Methods S1 for details).
