Ample human cells had engrafted 2 weeks post-transplantation as revealed by staining for human-specific nuclear antigen (HuNu) and EGFP (Supplementary Fig. 8). The majority of the cells remained close to the injection core and expressed the neuronal marker Doublecortin (DCX). By 3 months post-transplantation, most cells expressed the neuronal marker NeuN and GABA (Supplementary Fig. 8). We also noticed that NeuN expression increased over time, which suggested the continuous maturation of AD-iN cells in vivo. Similar to their differentiation in vitro, we observed SST, CR and CB expression in the grafted cells. We also detected suggestive colabeling of human cells with NPY and PV antibodies (Fig. 3f). Thus, GABAergic AD-iN cells are transgene independent and can stably integrate into the rodent brain, where they continue to mature.
