In the Replication and postmortem cohorts, methylation analysis on the same SLC6A4 proximal promoter 20 CpG sites as targeted by the ADS580FS1 and ADS580FS2 assays was carried out at the Core for Advanced Translational Technologies (UTHSCSA). The protocol used was the same as in the Discovery cohort, except with independently designed PCR and sequencing primers (primer sequences available upon request). Results were analyzed using PyroMark Q96 MD and PyroMark CpG 1.0 software (Qiagen, Valencia, CA).
