molecular barcoded probes (Sup. Table 6)37. Using this scorecard, we found no correlation between an iPSC line’s ability to establish NPCs and its predicted differentiation capacity into ectoderm-derived cell types or the expression of a broad set of endodermal and mesodermal markers (Sup. Fig. 9, 10). Taken together with the pluripotency marker analysis, teratoma formation assays and PluriTest assay results, these findings on all 12 of the iPSCs indicate that the BD-patient iPSCs do not exhibit a general differentiation defect that could otherwise confound the interpretation of the deficits in neural differentiation that we observed.
