This study aims to investigate the relationship of smoking to all-cause mortality in Australia, in the 45 and Up Study cohort. Although cause-specific mortality data have been used in analyses from other countries, these were not available for Australia at the time of writing. Participants in this population-based cohort study were predominantly born between 1920 and 1964, and have lived through the peak of the smoking epidemic, as well as through many changes in tobacco policy, legislation, and health information.