brain tissue. Expression data from the saline and ethanol treatment groups were background corrected, quantile normalized and summarized using the robust multi-array average (RMA) expression measure [25]. For analysis of SNPs possibly affecting microarray probe performance, the D2 genome sequence provided by Xusheng Wang in Dr. William's laboratory was used to identify probes overlapping a B6/D2 SNP (Table S8). Affymetrix probe sequences were aligned to the mm9 version of the mouse genome provided by Bioconductor, using the Biostrings package for R. All datasets generated for this paper can be queried on GeneNetwork (genenetwork.org) or downloaded in their entirety from the Gene Expression Omnibus repository under accession number GSE28515. All data is MIAME compliant.
