of 120000, RF lens% 30, maximum inject time 50 ms, standard AGC target, including charges of 2 to 6 for fragmentation with 60 sec dynamic exclusion. MS2 scans were performed with a fixed first mass of 100 m/z, 34% fixed CE, 50000 resolution, 20% normalized AGC target and dynamic maximum IT. The data were recorded using Thermo Fisher Scientific Xcalibur (4.3) software (Thermo Fisher Scientific Inc.).