DAGL catalyzes the second step of 2-AG synthesis, liberating 2-AG from diacylglycerol (see above). Recent molecular characterization of an Sn-1 selective DAGL has shed considerable light on the 2-AG-CB1 signaling system (Bisogno et al., 2003). Two isoforms, alpha and beta DAGL, were cloned and appear to have similar enzymatic properties. During embryonic development, DAGLα is localized to axon terminals, whereas during adulthood, a somatic/dendritic localization seems to predominate (Bisogno et al., 2003).
