Chunk #9 — MATERIALS AND METHODS — ANIMAL EXPERIMENTATION — Glucose tolerance test, insulin tolerance test, and pyruvate tolerance test

Source

CYP2A6 is associated with obesity: studies in human samples and a high fat diet mouse model.

Embedded

yes

Text

For Glucose tolerance test and pyruvate tolerance test, the mice were subjected to an overnight fast (15h) followed by glucose or pyruvate injection ip at 1 g/kg and 2 g/kg, respectively. For insulin tolerance test, under feeding status the mice were injected with insulin ip at 2 U/kg. Blood was collected from tails for measuring blood glucose by Bayer Contour blood glucose meter.