Zfhx1bF/F mice were genotyped according to (Miyoshi et al., 2006). CAG-CAT-eGFP mice were genotyped according to (Kawamoto et al., 2000). Zfhx1bF/F males were crossed to Beta-Actin Cre mice (Lewandoski et al., 1997) to generate the Zfhx1b null allele, which was followed by a cross to wildtype mice to eliminate the Beta-Actin Cre allele. Zfhx1b+/-mice were crossed with Nkx2.1-Cre I1/2b-Cre mice, and male Zfhx1b+/-; Cre+ mice were crossed with female Zfhx1bF/F mice with or without the CAG-CAT-EGFP allele to generate conditional mutant embryos.
