Chunk #10 — Materials and methods — RNA extraction

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Quality control parameters on a large dataset of regionally dissected human control brains for whole genome expression studies.

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After purification all RNA samples were applied to a RNA 6000 Nano-LabChip and analysed using the Agilent 2100 Bioanalyzer (Agilent Technologies UK Ltd) to obtain the RIN values. RINs and total RNA electropherograms were calculated by the 2100 Expert Software (Agilent Technologies UK Ltd). Further assessment of the RIN was performed by checking each electropherogram visually.