Chunk #18 — Experimental Design — gRNA and donor template design

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Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.

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Critical parameters that should be optimized for genome editing are the design of the gRNA and the HDR donor (Box 1). As we noted previously, SNPs not represented in the reference genome may lead to off-target sites not predicted by such design algorithms4.