We repeatedly exposed the SH-SY5Y cells to 40 mM ethanol for 4 h per day, 4 days per week over 3 weeks. This concentration is equivalent to a blood alcohol concentration of 0.18%, which can be reached by a 180-pound man drinking about 7 beers over 2 h, and is below the level defined as “high intensity drinking” (Hingson et al., 2017). We found that the expression of many genes was affected. We also examined the effect of a 24 h withdrawal after the last exposure, and found most of those genes returned toward baseline expression.
