The goal of the Allen Human Brain Atlas is to create a comprehensive map of transcript usage across the entire adult brain, with the emphasis on anatomically complete coverage at a fine nuclear resolution in a small number of high-quality, clinically unremarkable brains profiled with DNA microarrays for quantitative gene-level transcriptome coverage. Furthermore, structural brain imaging data were obtained from each individual to visualize gene expression data in its native three-dimensional anatomical coordinate space, and to allow correlations between imaging and transcriptome modalities. These data are freely accessible via the Allen Brain Atlas data portal (http://www.brain-map.org).