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Chunk #34 — Materials and Methods — Data preprocessing

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Network analysis of intrinsic functional brain connectivity in Alzheimer's disease.
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Data (rest1 and rest2 scans) were preprocessed using statistical parametric mapping (SPM2) software (http://fil.ion.ucl.ac.uk/spm). The first 8 image acquisitions of the task-free functional time series were discarded to allow for stabilization of the MR signal. Each of the remaining 172 volumes underwent the following preprocessing steps: realignment, normalization, and smoothing. Normalization was to the Montreal Neurological Institute (MNI) template and smoothing was done with a 4 mm full width half maximum Gaussian kernel to decrease spatial noise. Excessive motion, defined in our lab as greater than 3.5 mm of translation or 3.5 degrees of rotation in any plane, was not present in any of the task-free scans.