approach applied. In particular, the Salamanca group screened at least ten times more cells (5 million per case vs. 200,000 – 500,000) and used 8- color staining panels as compared to the previously used 4-color(5) and 5-color(8) protocols. The percentage of aberrant/clonal B cells was below the maximum sensitivity of the previous studies (<0.01%) in more than half of the cases (62%) identified by the Salamanca study.(9) However, it should be noted that clonality was only confirmed by additional methods in 18 cases, all except one with >0.01% aberrant/clonal B-cells.(9)
