Program 7M was used for two-group discriminant function analysis (DFA) [54-56]. Program 7M produces a new canonical variable, the discriminant function, which maximally separates two groups based on a weighted combination of entered variables. DFA defines the significance of a group separation, summarizes the classification of each participant, and provides an approach for the prospective classification of individuals not involved in discriminant rule generation or for classification of a new population. The analysis reports the significance of group separation statistically by Wilks’ lambda with Rao’s approximation. To estimate prospective classification success, the jackknifing technique, also referred to as the leaving-one-out process, was used [57,58]. By this method, discriminant function is formed on all individuals but one. The left-out individual is subsequently classified. This initial left out individual is then folded back into the group (hence ‘jackknifing’), and another individual is left out. The process is repeated until each individual has been left out and classified. The measure of classification success is then based upon a tally of the correct classifications of the left-out individuals.