Chunk #17 — MATERIALS AND METHODS — Genotyping — OPRM1

Source

Naltrexone modification of drinking effects in a subacute treatment and bar-lab paradigm: influence of OPRM1 and dopamine transporter (SLC6A3) genes.

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Genomic DNA was extracted from peripheral blood mononuclear cells using a commercial DNA extraction kit/procedure (Gentra Puragene Blood Kit, Qiagen Inc., Valencia CA). The Taqman 5′ nuclease genotyping assay was used for OPRM1 SNP analysis (Applied Biosystems (ABI), Foster City, CA).