We next annotated the enriched genes with KEGG in the early differentiation period from D0 to D7 (Table 1). From D0 to D7, the genes in the signaling pathways that regulate the general biological proliferation of “stem cells”, such as DNA replication, cell cycle, spliceosome, and others, were down regulated (Table 1), whereas the genes in the pathways that regulate neuronal maturity, including the MAPK signaling pathway, neuroactive ligand-receptor interaction, Wnt signaling pathway, and others were up-regulated (Table 1). The disorder groups were module 14 and module 1. In these groups, the modulate synapse and neuron projection in module 14 showed a pattern of increased expression from D0 to D28.Table 1Up and down regulated pathway enrichment by KEGG.RankMapIDMapTitlePvalueAdjustedPv Down regulated pathway from D0 to D7 enrichment by KEGG 1map03010Ribosome3.20E-315.70E-292map03040Spliceosome1.71E-161.52E-143map03030DNA replication6.09E-133.61E-114map03013RNA transport6.96E-122.38E-105map04110Cell cycle8.01E-122.38E-10 Up regulated pathway from D0 to D7 enrichment by KEGG 1map04010MAPK signaling pathway2.18E-163.40E-142map04020Calcium signaling pathway7.22E-153.75E-133map04070Phosphatidylinositolsignaling system4.67E-131.21E-114map04080Neuroactive ligand-receptor interaction4.99E-088.66E-075map04144Endocytosis3.83E-065.44E-056map04012ErbB signaling pathway9.47E-060.0001237map00562Inositol phosphate metabolism4.56E-050.0005088map04310Wnt signaling pathway0.0001680.001751 Down regulated pathway from D7 to D28 enrichment by KEGG 1map04080Neuroactive ligand-receptor interaction0.003960.178222232map00514O-Mannosyl glycan biosynthesis0.0108990.189657833map04020Calcium signaling pathway0.0126440.189657834map04310Wnt signaling pathway0.0357050.362217316 Up regulated pathway from D7
