triple-banding pattern (approx. 80–85 kDa), which was unchanged in response to rhuFGF21, suggesting basal activation of the pathway, proximal to ERK activation. We next interrogated the FRS2 adaptor Src homology domain 2 containing protein tyrosine phosphatase (SHP2) (Figure 3C). In Tg WAT, SHP2 was minimally phosphorylated when unstimulated and remained unchanged in response to rhuFGF21. In liver, rhuFGF21 elicited similar ERK phosphorylation in both WT and Tg animals (Figure 3D).
