Chunk #13 — Materials and Methods — Stereotaxic surgery and behavioral electrophysiology

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Encoding of the Intent to Drink Alcohol by the Prefrontal Cortex Is Blunted in Rats with a Family History of Excessive Drinking.

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Spike trains were manually identified and sorted into individual cell clusters based on the features of the wave form in Spike Sort 3D (Neuralynx). After cell sorting, duplicate timestamps and interspike intervals <3 ms were removed from spike trains. Only spike trains containing >150 spikes were analyzed.