Within the complex, the LPHN3-OLF flexible 316–329 loop is stabilized in the closed state, via direct interactions with FLRT3-LRR established by Asn316, Tyr317, Asp319 and Thr320. The mobility of the entire loop is further restrained by the interfacing interactions established by Arg376 with Tyr43 and Tyr64 in FLRT3-LRR, which maintains the β13-β14 connecting loop on top of the 316–329 loop and contributes to lock its conformation (Figure 5B,C).
