TAF15 full-length plasmid TAF15+, MTF1 full-length plasmid MTF1+, YY2 full-length plasmid YY2+, and their respective expression vectors (NCs) were designed and synthesized in GenScript (Nanjing, China), while LINC00665 full-length plasmid LINC00665+ and its NC were designed and synthesized in GeneChem (Shanghai, China). Short-hairpin LINC00665 (sh-LINC00665), MTF1 (sh-MTF1), YY2 (sh-YY2), GTSE1 (sh-GTSE1), STAU1 (sh-STAU1), and UPF1 (sh-UPF1) plasmids and their respective NCs (sh-NCs) were designed and synthesized in GenePharma (China) (Table 1). Cell transfections were performed as previously described. Briefly, cells were seeded into 24-well plates (Corning Life Sciences) and then transfected with the reagent Lipofectamine 3000 (Life Technologies, USA). G418 and puromycin were used to screen stable transfected cells.Table 1shRNA SequencesNameTarget Sequencesh-NC5′-GTTCTCCGAACGTGTCACGTC-3′sh-LINC006655′-GCGTCCACGGGTGGGAAATTG-3′sh-MTF15′-GGATACAAATCACTCACTTTG-3′sh-YY25′-GCCTCCAACGAAGATTTCTCC-3′sh-GTSE15′-GCCTACTCCTACAAATCAATT-3′sh-STAU15′-GCCGCAGGGAGTTTGTGATGC-3′sh-UPF15′-GCGAGAAGGACTTCATCATCC-3′
